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TOMM20-mTurquoise-RhoA-G14V-deltaCaaX
(Plasmid #176123)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176123 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RHOA
  • Species
    H. sapiens (human)
  • Mutation
    G14V
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TOMM20-mTurquoise-RhoA-G14V-deltaCaaX was a gift from Dorus Gadella (Addgene plasmid # 176123 ; http://n2t.net/addgene:176123 ; RRID:Addgene_176123)

  • For your References section:

    Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity. Mahlandt EK, Kreider-Letterman G, Chertkova AO, Garcia-Mata R, Goedhart J. J Cell Sci. 2023 May 15;136(10):jcs260802. doi: 10.1242/jcs.260802. Epub 2023 May 25. 10.1242/jcs.260802 PubMed 37226883
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