H2A-mTurquoise-RhoJQ79L-deltaCaax
(Plasmid
#176110)
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PurposeConstitutively active, nucelar localized RhoJ
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176110 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRhoJ
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SpeciesH. sapiens (human)
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MutationQ79L
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Entrez GeneRHOJ (a.k.a. ARHJ, RASL7B, TC10B, TCL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H2A-mTurquoise-RhoJQ79L-deltaCaax was a gift from Dorus Gadella (Addgene plasmid # 176110 ; http://n2t.net/addgene:176110 ; RRID:Addgene_176110) -
For your References section:
Cell-based optimization and characterization of genetically encoded location-based biosensors for Cdc42 or Rac activity. Mahlandt EK, Kreider-Letterman G, Chertkova AO, Garcia-Mata R, Goedhart J. J Cell Sci. 2023 May 15;136(10):jcs260802. doi: 10.1242/jcs.260802. Epub 2023 May 25. 10.1242/jcs.260802 PubMed 37226883