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Addgene

dimericTomato-2xrGBD
(Plasmid #176098)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176098 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rhotekin
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Rtkn
  • Promoter CMVdel

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dimericTomato-2xrGBD was a gift from Dorus Gadella (Addgene plasmid # 176098 ; http://n2t.net/addgene:176098 ; RRID:Addgene_176098)

  • For your References section:

    Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor. Mahlandt EK, Arts JJG, van der Meer WJ, van der Linden FH, Tol S, van Buul JD, Gadella TWJ, Goedhart J. J Cell Sci. 2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823. 10.1242/jcs.258823 PubMed 34357388
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