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pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro
(Plasmid #176089)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Lentivirus
  • Total vector size (bp) 10056
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PolB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1008
  • Mutation
    Mutations in Lys35 to Ala, Lys68 to Ala, and Lys72 to Ala, and mutation in the PAM site used by PolB gRNA1
  • Entrez Gene
    POLB
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EGFP-PolB-(K35A/K68A/K72A)-PAMmut-Hygro was a gift from Robert Sobol (Addgene plasmid # 176089 ; http://n2t.net/addgene:176089 ; RRID:Addgene_176089)

  • For your References section:

    Temporal dynamics of base excision/single-strand break repair protein complex assembly/disassembly are modulated by the PARP/NAD(+)/SIRT6 axis. Koczor CA, Saville KM, Andrews JF, Clark J, Fang Q, Li J, Al-Rahahleh RQ, Ibrahim M, McClellan S, Makarov MV, Migaud ME, Sobol RW. Cell Rep. 2021 Nov 2;37(5):109917. doi: 10.1016/j.celrep.2021.109917. 10.1016/j.celrep.2021.109917 PubMed 34731617
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