Skip to main content
Addgene

pET28 MBP_PrA/G-H6
(Plasmid #176077)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5273
  • Total vector size (bp) 6914
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MBP PrA/G-H6
  • Species
    bacteria
  • Insert Size (bp)
    1641
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • His tag (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28 MBP_PrA/G-H6 was a gift from Tom Rapoport (Addgene plasmid # 176077 ; http://n2t.net/addgene:176077 ; RRID:Addgene_176077)

  • For your References section:

    Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies). Wu X, Rapoport TA. Proc Natl Acad Sci U S A. 2021 Oct 12;118(41). pii: 2115001118. doi: 10.1073/pnas.2115001118. 10.1073/pnas.2115001118 PubMed 34620716
Related items:
Commonly requested with: