pLV-EF1A-LivePAR(Y107A)-Hygro
(Plasmid #176073)

• Purpose: EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette
• Depositing Lab: Robert Sobol
• Publication: Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi: 10.1016/j.celrep.2021.109917.

Backbone

• Vector backbone: Lentivirus
• Total vector size (bp): 9917
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LivePAR (Y107A)
• Alt name: WWE Domain
• Species: H. sapiens (human)
• Insert Size (bp): 993
• Mutation: Point mutation to convert Try107 to Ala
• Promoter: EF1A
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pLV-EF1A-LivePAR(Y107A)-EGFP-Hygro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry