pRK5-HA-Ubiquitin-K33
(Plasmid #17607)

• Purpose: Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines
• Depositing Lab: Ted Dawson
• Publication: Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Backbone

• Vector backbone: pRK5-HA
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin C
• Alt name: Ub
• Species: H. sapiens (human)
• Insert Size (bp): 220
• Mutation: K33 only, other lysines mutated to arginines; P19S and E24D
• Entrez Gene: UBC (a.k.a. HMG20)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified P19S and E24D mutations in the ubiquitin sequence. These mutations were not intentional, but were present in the original source clone from which this plasmid was created (see associated publication for details).