pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG
(Plasmid #176059)

• Purpose: Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.
• Depositing Lab: Britt Glaunsinger
• Publication: Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi: 10.1016/j.celrep.2021.109841. Epub 2021 Sep 30.

Backbone

• Vector backbone: pCDNA4TO
• Backbone size w/o insert (bp): 5163
• Total vector size (bp): 540
• Vector type: Mammalian Expression
• Selectable markers: Bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CoV-2 nsp1 R124A/K125A
• Alt name: RNA destabilization mutant of nsp1
• Species: SARS-CoV-2
• Insert Size (bp): 540
• Mutation: R124A and K125A
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry