pRK5-HA-Ubiquitin-KO
(Plasmid #17603)

• Purpose: Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)
• Depositing Lab: Ted Dawson
• Publication: Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Backbone

• Vector backbone: pRK5-HA
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin C
• Alt name: Ub
• Species: H. sapiens (human)
• Insert Size (bp): 220
• Mutation: KO: all lysines mutated to arginines
• Entrez Gene: UBC (a.k.a. HMG20)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 73 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The ubiquitin KO sequence in this plasmid contains the following lysine to arginine mutations: K6R, K11R, K27R, K29R, K33R, K48R and K63R.