pCDH-EF1a-tetR-T2A-iRFP
(Plasmid
#176028)
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Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176028 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH
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Backbone manufacturerSystem Biosciences
- Total vector size (bp) 8068
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nametetR
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SpeciesSynthetic
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Insert Size (bp)633
Gene/Insert 2
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Gene/Insert nameiRFP
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Insert Size (bp)950
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-EF1a-tetR-T2A-iRFP was a gift from Irmela Jeremias (Addgene plasmid # 176028 ; http://n2t.net/addgene:176028 ; RRID:Addgene_176028) -
For your References section:
Inducible transgene expression in PDX models in vivo identifies KLF4 as a therapeutic target for B-ALL. Liu WH, Mrozek-Gorska P, Wirth AK, Herold T, Schwarzkopf L, Pich D, Volse K, Melo-Narvaez MC, Carlet M, Hammerschmidt W, Jeremias I. Biomark Res. 2020 Sep 16;8:46. doi: 10.1186/s40364-020-00226-z. eCollection 2020. 10.1186/s40364-020-00226-z PubMed 32944247