pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP
(Plasmid
#176027)
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Purposeconstruct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH
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Backbone manufacturerSystem Biosciences
- Total vector size (bp) 10459
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namertTA3
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Insert Size (bp)747
Gene/Insert 2
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Gene/Insert nameLuc2
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SpeciesFirefly Luc
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Insert Size (bp)1650
Gene/Insert 3
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Gene/Insert namemtagBFP
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SpeciesSynthetic
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Insert Size (bp)706
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP was a gift from Irmela Jeremias (Addgene plasmid # 176027 ; http://n2t.net/addgene:176027 ; RRID:Addgene_176027) -
For your References section:
Inducible transgene expression in PDX models in vivo identifies KLF4 as a therapeutic target for B-ALL. Liu WH, Mrozek-Gorska P, Wirth AK, Herold T, Schwarzkopf L, Pich D, Volse K, Melo-Narvaez MC, Carlet M, Hammerschmidt W, Jeremias I. Biomark Res. 2020 Sep 16;8:46. doi: 10.1186/s40364-020-00226-z. eCollection 2020. 10.1186/s40364-020-00226-z PubMed 32944247