EGFP-Bin1b
(Plasmid
#176014)
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PurposeMammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176014 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSDEST2 (Addgene 22424)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBin1b
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SpeciesD. rerio (zebrafish)
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Entrez Genebin1b (a.k.a. bi, bin1, cb57, zgc:86701)
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-Bin1b was a gift from Rob Parton (Addgene plasmid # 176014 ; http://n2t.net/addgene:176014 ; RRID:Addgene_176014) -
For your References section:
Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle. Lo HP, Lim YW, Xiong Z, Martel N, Ferguson C, Ariotti N, Giacomotto J, Rae J, Floetenmeyer M, Moradi SV, Gao Y, Tillu VA, Xia D, Wang H, Rahnama S, Nixon SJ, Bastiani M, Day RD, Smith KA, Palpant NJ, Johnston WA, Alexandrov K, Collins BM, Hall TE, Parton RG. J Cell Biol. 2021 Dec 6;220(12). pii: 212693. doi: 10.1083/jcb.201905065. Epub 2021 Oct 11. 10.1083/jcb.201905065 PubMed 34633413