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Addgene

pUC8 human IFN-gamma
(Plasmid #17600)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17600 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC8
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 2665
  • Modifications to backbone
    Modified backbone--contains T7 promoter; does not contain M13 priming sites normally present in pUC8
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    interferon-gamma cDNA
  • Alt name
    IFN-gamma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    IFNG (a.k.a. IFG, IFI, IMD69)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvaI (not destroyed)
  • 3′ cloning site HincII (not destroyed)
  • 5′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IFN-gamma cDNA. Young lab stock #D2994.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC8 human IFN-gamma was a gift from Howard Young (Addgene plasmid # 17600 ; http://n2t.net/addgene:17600 ; RRID:Addgene_17600)

  • For your References section:

    Mucosa-specific targets for regulation of IFN-gamma expression: lamina propria T cells use different cis-elements than peripheral blood T cells to regulate transactivation of IFN-gamma expression. Gonsky R, Deem RL, Bream JH, Lee DH, Young HA, Targan SR. J Immunol. 2000 Feb 1. 164(3):1399-407. PubMed 10640755
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