pBEL2414
(Plasmid
#175966)
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PurposeGenetic complementation plasmid of LetAB with LetB(P701N)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET17b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3174
- Total vector size (bp) 7073
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameLetA
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Alt nameyebS
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Alt nameb1833
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Alt nameECK1832
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SpeciesE. coli
- Promoter T7
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameLetB
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Alt nameyebT
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Alt nameb1834
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Alt nameECK1833
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SpeciesE. coli
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MutationP701N
- Promoter T7
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEL2414 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 175966 ; http://n2t.net/addgene:175966 ; RRID:Addgene_175966) -
For your References section:
Role of Ring6 in the Function of the E. coli MCE Protein LetB. Vieni C, Coudray N, Isom GL, Bhabha G, Ekiert DC. J Mol Biol. 2022 Jan 22;434(7):167463. doi: 10.1016/j.jmb.2022.167463. 10.1016/j.jmb.2022.167463 PubMed 35077766