pBEL2265
(Plasmid #175962)

• Purpose: For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
• Depositing Labs: Gira Bhabha, Damian Ekiert
• Publication: Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. doi: 10.1016/j.jmb.2022.167463.

Backbone

• Vector backbone: pBAD-His
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 4571
• Total vector size (bp): 8664
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: LetA
• Alt name: yebS
• Alt name: b1833
• Alt name: ECK1832
• Species: E. coli
• Promoter: araBAD
• Tag / Fusion Protein:
  • 8xHis 2xQH and TEV cleavage site on LetA (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBAD fwd
• 3′ sequencing primer: pBAD rev

Gene/Insert 2

• Gene/Insert name: LetB
• Alt name: yebT
• Alt name: b1834
• Alt name: ECK1833
• Species: E. coli
• Mutation: Δ347-361, replace with 702-716
• Promoter: araBAD

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBAD fwd
• 3′ sequencing primer: pBAD rev

Resource Information

• Supplemental Documents:
  • pBEL2265.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry