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pBEL2265
(Plasmid #175962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD-His
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 4571
  • Total vector size (bp) 8664
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LetA
  • Alt name
    yebS
  • Alt name
    b1833
  • Alt name
    ECK1832
  • Species
    E. coli
  • Promoter araBAD
  • Tag / Fusion Protein
    • 8xHis 2xQH and TEV cleavage site on LetA (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    LetB
  • Alt name
    yebT
  • Alt name
    b1834
  • Alt name
    ECK1833
  • Species
    E. coli
  • Mutation
    Δ347-361, replace with 702-716
  • Promoter araBAD

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEL2265 was a gift from Gira Bhabha & Damian Ekiert (Addgene plasmid # 175962 ; http://n2t.net/addgene:175962 ; RRID:Addgene_175962)

  • For your References section:

    Role of Ring6 in the Function of the E. coli MCE Protein LetB. Vieni C, Coudray N, Isom GL, Bhabha G, Ekiert DC. J Mol Biol. 2022 Jan 22;434(7):167463. doi: 10.1016/j.jmb.2022.167463. 10.1016/j.jmb.2022.167463 PubMed 35077766
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