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HisMS2_VLP_RSV
(Plasmid #175958)

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Item Catalog # Description Quantity Price (USD)
Plasmid 175958 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pMC037-HisMS2_PLP_pac
  • Backbone size w/o insert (bp) 4630
  • Total vector size (bp) 6491
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    P gene
  • Species
    Respiratory syncytial virus
  • Insert Size (bp)
    914
  • GenBank ID
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Esp3I (BsmBI) (destroyed during cloning)
  • 3′ cloning site Esp3I (BsmBI) (destroyed during cloning)
  • 5′ sequencing primer TGTCGAACAGAAAGTAATCGTATTGTAC
  • 3′ sequencing primer CAGCTGGCACGACAGGTTTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    M gene
  • Species
    Respiratory syncytial virus
  • Insert Size (bp)
    958
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Esp3I (BsmBI) (destroyed during cloning)
  • 3′ cloning site Esp3I (BsmBI) (destroyed during cloning)
  • 5′ sequencing primer TGTCGAACAGAAAGTAATCGTATTGTAC
  • 3′ sequencing primer CAGCTGGCACGACAGGTTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.04.17.24305965 for medRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HisMS2_VLP_RSV was a gift from Paul Freemont (Addgene plasmid # 175958 ; http://n2t.net/addgene:175958 ; RRID:Addgene_175958)

  • For your References section:

    A standardised, high-throughput approach to diagnostic group testing method validation. Unruh LAH, Crone MA, Freemont PS, Chindelevitch L. medRxiv 2024.04.17.24305965 10.1101/2024.04.17.24305965
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