pDest490-OPN-10 kDa
(Plasmid
#17595)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDest490
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5300
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOPN-10 kDa
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Alt nameOsteopontin
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Alt nameOPN
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SpeciesH. sapiens (human)
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Insert Size (bp)132
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MutationBase pairs 499–630
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GenBank IDX13694
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Entrez GeneSPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
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Tag
/ Fusion Protein
- Flag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Called OPN-5 kDa in the reference.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDest490-OPN-10 kDa was a gift from Xin Wang (Addgene plasmid # 17595 ; http://n2t.net/addgene:17595 ; RRID:Addgene_17595) -
For your References section:
An osteopontin fragment is essential for tumor cell invasion in hepatocellular carcinoma. Takafuji V, Forgues M, Unsworth E, Goldsmith P, Wang XW. Oncogene. 2007 Sep 27. 26(44):6361-71. 10.1038/sj.onc.1210463 PubMed 17452979