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pET28_6xHis-GAL4-PARP1-CAT-L713F
(Plasmid #175949)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175949 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5360
  • Total vector size (bp) 6900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protein expression may be toxic to host, add 10 mM benzamide to all growth media, see PMID: 28695525 for protocol)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GAL4 DNA binding domain
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1500
  • Mutation
    L713F, V762A for the 'PARP1-CAT-L713F' portion
  • GenBank ID
  • Entrez Gene
    GAL4 (a.k.a. YPL248C, GAL81)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis tag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer T7 promoter primer
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28_6xHis-GAL4-PARP1-CAT-L713F was a gift from Joan Conaway (Addgene plasmid # 175949 ; http://n2t.net/addgene:175949 ; RRID:Addgene_175949)

  • For your References section:

    Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling. Ooi SK, Sato S, Tomomori-Sato C, Zhang Y, Wen Z, Banks CAS, Washburn MP, Unruh JR, Florens L, Conaway RC, Conaway JW. Proc Natl Acad Sci U S A. 2021 Sep 7;118(36). pii: 2107277118. doi: 10.1073/pnas.2107277118. 10.1073/pnas.2107277118 PubMed 34465625
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