CKAP2-mNeonGreen pHAT
(Plasmid #175947)

• Purpose: Bacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagII
• Depositing Lab: Susanne Bechstedt
• Publication: McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi: 10.7554/eLife.72202.

Backbone

• Vector backbone: pHAT2
• Backbone manufacturer: Peränen J., Rikkonen M.,Hyvönen M., Kääriäinen L. (1996) T7 vectors with modified T7lac promoter for expression of proteins in E
• Backbone size w/o insert (bp): 4476
• Total vector size (bp): 7197
• Modifications to backbone: C-terminal mNeonGreen and StrepTagII
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CKAP2
• Alt name: LB1, TMAP
• Species: M. musculus (mouse)
• Insert Size (bp): 1992
• GenBank ID: NM_001004140.2
• Entrez Gene: Ckap2 (a.k.a. AI461656, AW228814, LB1)
• Promoter: T7
• Tags / Fusion Proteins:
  • mNeonGreen (C terminal on backbone)
  • StrepTagII (C terminal on backbone)
  • 6His (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6, mNeonGreen_N

Resource Information

• Supplemental Documents:
  • mmCKAP2_pHAT-HUNS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry