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pDest490-OPN-c
(Plasmid #17592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDest490
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OPN-c
  • Alt name
    Osteopontin
  • Alt name
    OPN
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    860
  • Mutation
    Base pairs 1–93, 175–942
  • GenBank ID
    X13694
  • Entrez Gene
    SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

OPN-c is missing the amino-terminus exon 4 (27 amino acids).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest490-OPN-c was a gift from Xin Wang (Addgene plasmid # 17592 ; http://n2t.net/addgene:17592 ; RRID:Addgene_17592)

  • For your References section:

    An osteopontin fragment is essential for tumor cell invasion in hepatocellular carcinoma. Takafuji V, Forgues M, Unsworth E, Goldsmith P, Wang XW. Oncogene. 2007 Sep 27. 26(44):6361-71. 10.1038/sj.onc.1210463 PubMed 17452979
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