pDest490-OPN-b
(Plasmid #17591)

• Depositing Lab: Xin Wang
• Publication: Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Backbone

• Vector backbone: pDest490
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OPN-b
• Alt name: Osteopontin
• Alt name: OPN
• Species: H. sapiens (human)
• Insert Size (bp): 900
• Mutation: Base pairs 1–175, 217–942; See Depositor Comments
• GenBank ID: X13694
• Entrez Gene: SPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
• Tag / Fusion Protein:
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC results detected a D102G mutation in the insert. This variant was present in the original sample and should not alter the function of the plasmid as described in the associated publication.