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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDest-490
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5300
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOPN-a
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Alt nameOPN-FL
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Alt nameOsteopontin
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SpeciesH. sapiens (human)
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Insert Size (bp)897
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MutationBase pairs 49–942
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GenBank IDX13694
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Entrez GeneSPP1 (a.k.a. BNSP, BSPI, ETA-1, OPN)
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Tag
/ Fusion Protein
- Flag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mature human OPN cDNA.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDest490-OPN-a was a gift from Xin Wang (Addgene plasmid # 17590 ; http://n2t.net/addgene:17590 ; RRID:Addgene_17590) -
For your References section:
An osteopontin fragment is essential for tumor cell invasion in hepatocellular carcinoma. Takafuji V, Forgues M, Unsworth E, Goldsmith P, Wang XW. Oncogene. 2007 Sep 27. 26(44):6361-71. 10.1038/sj.onc.1210463 PubMed 17452979