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CG4845/pMT/V5-HisB
(Plasmid #17589)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17589 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT/V5-His B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CG4845
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    3000
  • GenBank ID
    NM_142601
  • Entrez Gene
    psidin (a.k.a. Dmel_CG4845, CG4845, Dmel\CG4845, ird16)
  • Tags / Fusion Proteins
    • 6x His (C terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer MT-Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CG4845/pMT/V5-HisB was a gift from Denise Montell (Addgene plasmid # 17589 ; http://n2t.net/addgene:17589 ; RRID:Addgene_17589)
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