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DDX41_p.L237F_p.P238T_EGFP
(Plasmid #175845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175845 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 6217
  • Total vector size (bp) 7981
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX41
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1866
  • Mutation
    c.711G>T, p.L237F and c.712C>A, p.P238T
  • GenBank ID
    NM_001321732.2
  • Entrez Gene
    DDX41 (a.k.a. ABS, MPLPF)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Age1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EGFP-N reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DDX41_p.L237F_p.P238T_EGFP was a gift from Evan Braunstein (Addgene plasmid # 175845 ; http://n2t.net/addgene:175845 ; RRID:Addgene_175845)

  • For your References section:

    Two novel germline DDX41 mutations in a family with inherited myelodysplasia/acute myeloid leukemia. Li R, Sobreira N, Witmer PD, Pratz KW, Braunstein EM. Haematologica. 2016 Jun;101(6):e228-31. doi: 10.3324/haematol.2015.139790. Epub 2016 Mar 4. 10.3324/haematol.2015.139790 PubMed 26944477
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