pFX-mCherry-KRasCT
(Plasmid
#175835)
-
PurposeExpress mCherry with plasma membrane-targeting sequence in mammalian cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 175835 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepFX
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4830
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemCherry with plasma membrane-targeting sequence
-
Insert Size (bp)830
-
Tag
/ Fusion Protein
- C-terminal hypervariable region of K-Ras 4B (C terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFX-mCherry-KRasCT was a gift from Yusuke Ohba (Addgene plasmid # 175835 ; http://n2t.net/addgene:175835 ; RRID:Addgene_175835) -
For your References section:
Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Kashiwagi S, Fujioka Y, Satoh AO, Yoshida A, Fujioka M, Nepal P, Tsuzuki A, Aoki O, Paudel S, Sasajima H, Ohba Y. Cell Struct Funct. 2019 Dec 26;44(2):183-194. doi: 10.1247/csf.19028. Epub 2019 Nov 15. 10.1247/csf.19028 PubMed 31735740