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pENTR4 CDS A. thaliana IMPORTIN ALPHA 3 / MOS6
(Plasmid #175820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR4
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 2243
  • Total vector size (bp) 3836
  • Vector type
    pENTR plasmid for Gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IMPORTIN ALPHA ISOFORM 3
  • Alt name
    AT4G02150.1
  • Alt name
    MODIFIER OF SNC1 6 (MOS6)
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1593
  • GenBank ID
    NM_116447
  • Entrez Gene
    MOS6 (a.k.a. AT4G02150, 6, ATIMPALPHA3, IMPA-3, IMPORTIN ALPHA 3, IMPORTIN ALPHA ISOFORM 3, MODIFIER OF SNC1, T10M13.16, T10M13_16)
  • Promoter none
  • Tags / Fusion Proteins
    • none
    • none

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCCGCCATAAACTGCCAGG
  • 3′ sequencing primer CGTTGAATATGGCTCATAACACCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR4 CDS A. thaliana IMPORTIN ALPHA 3 / MOS6 was a gift from Lennart Wirthmueller (Addgene plasmid # 175820 ; http://n2t.net/addgene:175820 ; RRID:Addgene_175820)

  • For your References section:

    Functional requirement of the Arabidopsis importin-alpha nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1. Ludke D, Roth C, Kamrad SA, Messerschmidt J, Hartken D, Appel J, Hornich BF, Yan Q, Kusch S, Klenke M, Gunkel A, Wirthmueller L, Wiermer M. Plant J. 2021 Feb;105(4):994-1009. doi: 10.1111/tpj.15082. Epub 2020 Dec 25. 10.1111/tpj.15082 PubMed 33210758
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