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pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2
(Plasmid #175816)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175816 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/D-Topo
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 4185
  • Vector type
    pENTR plasmid for Gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IMPORTIN ALPHA ISOFORM 2
  • Alt name
    AT4G16143.1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1605
  • Mutation
    natural polymorphism P65S, annotated in Genbank file
  • Entrez Gene
    IMPA-2 (a.k.a. AT4G16143, importin alpha isoform 2)
  • Promoter none
  • Tags / Fusion Proteins
    • none
    • none

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2 was a gift from Mark Banfield & Lennart Wirthmueller (Addgene plasmid # 175816 ; http://n2t.net/addgene:175816 ; RRID:Addgene_175816)

  • For your References section:

    Probing formation of cargo/importin-alpha transport complexes in plant cells using a pathogen effector. Wirthmueller L, Roth C, Fabro G, Caillaud MC, Rallapalli G, Asai S, Sklenar J, Jones AM, Wiermer M, Jones JD, Banfield MJ. Plant J. 2015 Jan;81(1):40-52. doi: 10.1111/tpj.12691. Epub 2014 Nov 17. 10.1111/tpj.12691 PubMed 25284001
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