pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1
(Plasmid #175814)

• Purpose: pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tags
• Depositing Labs: Mark Banfield, Lennart Wirthmueller
• Publication: Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1111/tpj.12691. Epub 2014 Nov 17.

Backbone

• Vector backbone: pENTR/D-Topo
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 2580
• Total vector size (bp): 5574
• Vector type: pENTR plasmid for Gateway cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IMPORTIN ALPHA ISOFORM 1
• Alt name: AT3G06720
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2994
• GenBank ID: NM_116472.4
• Entrez Gene: IMPA-1 (a.k.a. AT3G06720, AIMP ALPHA, AT-IMP, ATKAP ALPHA, IMPA1, IMPORTIN ALPHA, IMPORTIN ALPHA ISOFORM 1, importin alpha isoform 1)
• Promoter: none
• Tags / Fusion Proteins:
  • none
  • none

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13fw
• 3′ sequencing primer: M13rv

Resource Information

• Supplemental Documents:
  • pENTR_D-Topo genomic IMP1 (no stop).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry