pCSIIhyg-RGECO1.0
(Plasmid
#175813)
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PurposeThe lentiviral vector for R-GECO1.0, a genetically encoded red fluorescent Ca2+ sensor.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175813 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCSIIhyg
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRGECO1.0
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SpeciesSynthetic
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Insert Size (bp)1306
Resource Information
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A portion of this plasmid was derived from a plasmid made bycDNA coding RGECO1 was a gift from Dr. Robert Campbell (Addgene plasmid # 32444 ; http://n2t.net/addgene:32444 ; RRID:Addgene_32444). pCSII plasmid was developed by the late Dr. Miyoshi (RIKEN BioResource Center).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSIIhyg-RGECO1.0 was a gift from Michiyuki Matsuda (Addgene plasmid # 175813 ; http://n2t.net/addgene:175813 ; RRID:Addgene_175813) -
For your References section:
C-type Natriuretic Peptide-induced PKA Activation Promotes Endochondral Bone Formation in Hypertrophic Chondrocytes. Hirota K, Hirashima T, Horikawa K, Yasoda A, Matsuda M. Endocrinology. 2022 Mar 1;163(3). pii: 6511000. doi: 10.1210/endocr/bqac005. 10.1210/endocr/bqac005 PubMed 35041746