pCSIIpuro-4493NES
(Plasmid #175811)

• Purpose: The lentiviral vector for Booster-PKA, a Red-Shifted genetically encoded FRET biosensor for monitoring PKA activity.
• Depositing Lab: Michiyuki Matsuda
• Publication: Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941.

Backbone

• Vector backbone: pCSIIpuro
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Booster-PKA
• Species: Synthetic
• Insert Size (bp): 2600

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCAGGS-4493NES (Addgene #138373). pCSII plasmid was developed by the late Dr. Miyoshi (RIKEN BioResource Center).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The linker region between mKate2 and mKO2 is extremely repeat rich and difficult to validate using Sanger or short read NGS. Requesting scientists should validate expected length using restriction digests in addition to sequencing methods and functionality prior to experimental use.