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pET29b-dspB(E184Q)-6xHis
(Plasmid #175802)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET29b
  • Backbone size w/o insert (bp) 5316
  • Total vector size (bp) 6420
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Dispersin B
  • Alt name
    DspB
  • Species
    Aggregatibacter actinomycetemcomitans
  • Insert Size (bp)
    1104
  • Mutation
    E184Q
  • GenBank ID
    AAP31025
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistidine tag (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Prof. Narayanan Ramasubbu, Department of Oral Biology, University of Medicine and Dentistry of New Jersey

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29b-dspB(E184Q)-6xHis was a gift from Mark Nitz (Addgene plasmid # 175802 ; http://n2t.net/addgene:175802 ; RRID:Addgene_175802)

  • For your References section:

    An Inactive Dispersin B Probe for Monitoring PNAG Production in Biofilm Formation. Eddenden A, Kitova EN, Klassen JS, Nitz M. ACS Chem Biol. 2020 May 15;15(5):1204-1211. doi: 10.1021/acschembio.9b00907. Epub 2020 Jan 21. 10.1021/acschembio.9b00907 PubMed 31917539
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