Skip to main content
Addgene

SH2-free
(Plasmid #175800)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET15HE
  • Backbone manufacturer
    Stoddard Lab
  • Backbone size w/o insert (bp) 5727
  • Total vector size (bp) 6005
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Nck2 SH2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    297
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dr. Theresia Stradal of Helmholtz Centre for Infection Research

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene was cloned from pGEX-6P1:huNck2-SH2 plasmid generously provided by Dr. Theresia Stradal of Helmholtz Centre for Infection Research

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SH2-free was a gift from Barry Stoddard (Addgene plasmid # 175800 ; http://n2t.net/addgene:175800 ; RRID:Addgene_175800)

  • For your References section:

    Design of functionalised circular tandem repeat proteins with longer repeat topologies and enhanced subunit contact surfaces. Hallinan JP, Doyle LA, Shen BW, Gewe MM, Takushi B, Kennedy MA, Friend D, Roberts JM, Bradley P, Stoddard BL. Commun Biol. 2021 Oct 29;4(1):1240. doi: 10.1038/s42003-021-02766-y. 10.1038/s42003-021-02766-y PubMed 34716407
Related items: