Doughnut (DNT)
(Plasmid
#17580)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17580 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCRII
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3932
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namednt
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Alt namedoughnut
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SpeciesD. melanogaster (fly)
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GenBank IDAF123572
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Entrez Genednt (a.k.a. Dmel_CG17559, CG17559, DNT, Dmel\CG17559)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Doughnut (DNT) was a gift from Denise Montell (Addgene plasmid # 17580 ; http://n2t.net/addgene:17580 ; RRID:Addgene_17580) -
For your References section:
A Drosophila derailed homolog, doughnut, expressed in invaginating cells during embryogenesis. Savant-Bhonsale S, Friese M, McCoon P, Montell DJ. Gene. 1999 Apr 29. 231(1-2):155-61. 10.1016/S0378-1119(99)00061-X PubMed 10231580