tcTRP24sub8_SS
(Plasmid
#175798)
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Purposede novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing disulfide 'staples'
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET15HE
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Backbone manufacturerStoddard Lab
- Backbone size w/o insert (bp) 5727
- Total vector size (bp) 7088
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametcTRP24sub8_SS
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SpeciesSynthetic
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Insert Size (bp)1380
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tcTRP24sub8_SS was a gift from Barry Stoddard (Addgene plasmid # 175798 ; http://n2t.net/addgene:175798 ; RRID:Addgene_175798) -
For your References section:
Design of functionalised circular tandem repeat proteins with longer repeat topologies and enhanced subunit contact surfaces. Hallinan JP, Doyle LA, Shen BW, Gewe MM, Takushi B, Kennedy MA, Friend D, Roberts JM, Bradley P, Stoddard BL. Commun Biol. 2021 Oct 29;4(1):1240. doi: 10.1038/s42003-021-02766-y. 10.1038/s42003-021-02766-y PubMed 34716407