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pNL-EGFP/CMV/WPREdU3
(Plasmid #17579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 8998
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    761
  • GenBank ID
    U55763

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI, AfeI, AgeI (unknown if destroyed)
  • 3′ cloning site XhoI, BsrGI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNL-EGFP/CMV/WPREdU3 was a gift from Jakob Reiser (Addgene plasmid # 17579 ; http://n2t.net/addgene:17579 ; RRID:Addgene_17579)

  • For your References section:

    Optimized lentiviral transduction of mouse bone marrow-derived mesenchymal stem cells. Ricks DM, Kutner R, Zhang XY, Welsh DA, Reiser J. Stem Cells Dev. 2008 Jun . 17(3):441-50. 10.1089/scd.2007.0194 PubMed 18513160
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