pSAB35
(Plasmid #175787)

• Purpose: Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag
• Depositing Lab: John Rubinstein
• Publication: Abbas et al Science. 2020 Mar 13;367(6483):1240-1246. doi: 10.1126/science.aaz2924.

Backbone

• Vector backbone: pET28a(+)
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7102
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SidK (1-278)
• Species: L. pneumophila
• Insert Size (bp): 945
• Tags / Fusion Proteins:
  • 6xHis TEV (N terminal on insert)
  • 3xFLAG (C terminal on insert)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSAB35 was generated by inserting a 3xFLAG tag and a stop codon after residue 278 of full length SidK(1-573) in pET28a(+). There remains a second insert encoding SidK(279 - 573), without any tags, in pSAB35.