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pFastBac-GST-DAXX-His
(Plasmid #175781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac-GST
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    death domain associated protein
  • Species
    H. sapiens (human)
  • Entrez Gene
    DAXX (a.k.a. BING2, DAP6, EAP1, SMIM40)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • TEV (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pFastBac 5'
  • 3′ sequencing primer pFastBac 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-GST-DAXX-His was a gift from Xiaolu Yang (Addgene plasmid # 175781 ; http://n2t.net/addgene:175781 ; RRID:Addgene_175781)

  • For your References section:

    DAXX represents a new type of protein-folding enabler. Huang L, Agrawal T, Zhu G, Yu S, Tao L, Lin J, Marmorstein R, Shorter J, Yang X. Nature. 2021 Aug 18. pii: 10.1038/s41586-021-03824-5. doi: 10.1038/s41586-021-03824-5. 10.1038/s41586-021-03824-5 PubMed 34408321
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