pAC150-RFP-EGFP-LGALS3 R186S
(Plasmid
#175779)
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PurposePiggyBac Vector expressing GFP-RFP-LGALS3 R186S
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175779 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAC150
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLGALS3
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SpeciesH. sapiens (human)
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MutationR186S
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Entrez GeneLGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAC150-RFP-EGFP-LGALS3 R186S was a gift from Wade Harper (Addgene plasmid # 175779 ; http://n2t.net/addgene:175779 ; RRID:Addgene_175779) -
For your References section:
Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy. Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW. eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328. 10.7554/eLife.72328 PubMed 34585663