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Addgene

pAC150-RFP-EGFP-LGALS3 R186S
(Plasmid #175779)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175779 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAC150
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LGALS3
  • Species
    H. sapiens (human)
  • Mutation
    R186S
  • Entrez Gene
    LGALS3 (a.k.a. CBP35, GAL3, GALBP, GALIG, L31, LGALS2, MAC2)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC150-RFP-EGFP-LGALS3 R186S was a gift from Wade Harper (Addgene plasmid # 175779 ; http://n2t.net/addgene:175779 ; RRID:Addgene_175779)
  • For your References section:

    Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy. Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW. eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328. 10.7554/eLife.72328 PubMed 34585663