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Addgene

pHAGE-eGFP-OPTN S473A 513A
(Plasmid #175763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE-eGFP
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OPTN
  • Species
    H. sapiens (human)
  • Mutation
    S473A S513A
  • Entrez Gene
    OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-eGFP-OPTN S473A 513A was a gift from Wade Harper (Addgene plasmid # 175763 ; http://n2t.net/addgene:175763 ; RRID:Addgene_175763)

  • For your References section:

    Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy. Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW. eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328. 10.7554/eLife.72328 PubMed 34585663
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