pLTR-RD114A
(Plasmid #17576)

• Depositing Lab: Jakob Reiser
• Publication: Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Backbone

• Vector backbone: pEGFP-C1 (Clontech)
• Backbone size w/o insert (bp): 3417
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RD114 envelope glycoprotein
• Alt name: hybrid RD114 Env with cytoplasmic tail domain derived from the MLV 4070A Env
• Species: RD114 retrovirus
• Insert Size (bp): 2146
• GenBank ID: NC_009889

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Yasu Takeuchi
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Use packaging construct encoding Tat

see Shimode et al. Virus Genes 45, 393-397 (2012) for details regarding discrepancies in accession number X87829. See Accession number NC_009889.