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PurposeLentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175749 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNDP52/CALCOCO2
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SpeciesH. sapiens (human)
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Entrez GeneCALCOCO2 (a.k.a. NDP52)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-eGFP-NDP52 was a gift from Wade Harper (Addgene plasmid # 175749 ; http://n2t.net/addgene:175749 ; RRID:Addgene_175749) -
For your References section:
The PINK1-PARKIN Mitochondrial Ubiquitylation Pathway Drives a Program of OPTN/NDP52 Recruitment and TBK1 Activation to Promote Mitophagy. Heo JM, Ordureau A, Paulo JA, Rinehart J, Harper JW. Mol Cell. 2015 Oct 1;60(1):7-20. doi: 10.1016/j.molcel.2015.08.016. Epub 2015 Sep 10. 10.1016/j.molcel.2015.08.016 PubMed 26365381