pFIP
(Plasmid
#17565)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD18
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameinversion switch
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Alt namefimE
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SpeciesE. coli
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFIP was a gift from Jay Keasling (Addgene plasmid # 17565 ; http://n2t.net/addgene:17565 ; RRID:Addgene_17565) -
For your References section:
A tightly regulated inducible expression system utilizing the fim inversion recombination switch. Ham TS, Lee SK, Keasling JD, Arkin AP. Biotechnol Bioeng. 2006 May 5. 94(1):1-4. 10.1002/bit.20916 PubMed 16534780
Map uploaded by the depositor.
