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Addgene

pRelA'
(Plasmid #175595)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175595 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBbS2k
  • Backbone manufacturer
    BglBrick
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Catalytic domain of E.coli relA gene.
  • Alt name
    relA'
  • Species
    E. coli
  • Insert Size (bp)
    1365
  • Mutation
    Only the catalytic domain of the enzyme is used (C Terminal Domain [CTD] of WT RelA is removed). AGGAGGAAAAAA RBS sequence added before the start codon.
  • GenBank ID
    947244

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    RelA' is cloned from E.coli genomic DNA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRelA' was a gift from Greg Bokinsky (Addgene plasmid # 175595 ; http://n2t.net/addgene:175595 ; RRID:Addgene_175595)
  • For your References section:

    ppGpp is a bacterial cell size regulator. Buke F, Grilli J, Cosentino Lagomarsino M, Bokinsky G, Tans SJ. Curr Biol. 2022 Feb 28;32(4):870-877.e5. doi: 10.1016/j.cub.2021.12.033. Epub 2022 Jan 5. 10.1016/j.cub.2021.12.033 PubMed 34990598