pCFP
(Plasmid
#175593)
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PurposeLac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175593 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBbA5a
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCFP (cerulean)
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SpeciesAequorea victoria
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Insert Size (bp)717
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MutationAn improved monomeric CFP. See doi: 10.1038/nbt945
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byYFP was cloned from the Addgene plasmid mVenus N1, Plasmid #27793
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFP was a gift from Greg Bokinsky (Addgene plasmid # 175593 ; http://n2t.net/addgene:175593 ; RRID:Addgene_175593) -
For your References section:
ppGpp is a bacterial cell size regulator. Buke F, Grilli J, Cosentino Lagomarsino M, Bokinsky G, Tans SJ. Curr Biol. 2022 Feb 28;32(4):870-877.e5. doi: 10.1016/j.cub.2021.12.033. Epub 2022 Jan 5. 10.1016/j.cub.2021.12.033 PubMed 34990598