pRelA*
(Plasmid #175590)

• Purpose: Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
• Depositing Lab: Greg Bokinsky
• Publication: Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5. doi: 10.1016/j.cub.2021.12.033. Epub 2022 Jan 5.

Backbone

• Vector backbone: pBbS2k
• Backbone manufacturer: BglBrick
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Catalytic domain of E.coli relA gene.
• Alt name: relA'
• Species: E. coli
• Insert Size (bp): 1365
• Mutation: Only the catalytic domain of the enzyme is used (C Terminal Domain [CTD] of WT RelA is removed). AGGAGGAAAAAA RBS sequence added before the start codon.
• GenBank ID: 947244
• Tag / Fusion Protein:
  • Fused with a flexible GS linker to mVenus (C terminal on insert)

Resource Information

• Supplemental Documents:
  • sc101-KanR_sRBS_RelA'_YFP.gb
• A portion of this plasmid was derived from a plasmid made by: RelA* is a fusion of RelA'_YFP, RelA' is cloned from E.coli genomic DNA, YFP was cloned from the Addgene plasmid mVenus N1, Plasmid #27793

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry