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pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066
(Plasmid #175587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pALTER
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2462
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBM8A-3'UTR AluY-Sz WT
  • Insert Size (bp)
    1467
  • GenBank ID
    9939
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Acc1 (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSP72-RBM8A-3'UTR AluY-Sz WT 2598-4066 was a gift from Emanuela Felley-Bosco (Addgene plasmid # 175587 ; http://n2t.net/addgene:175587 ; RRID:Addgene_175587)

  • For your References section:

    Double-Stranded RNA Structural Elements Holding the Key to Translational Regulation in Cancer: The Case of Editing in RNA-Binding Motif Protein 8A. Abukar A, Wipplinger M, Hariharan A, Sun S, Ronner M, Sculco M, Okonska A, Kresoja-Rakic J, Rehrauer H, Qi W, Beusechem VWV, Felley-Bosco E. Cells. 2021 Dec 15;10(12). pii: cells10123543. doi: 10.3390/cells10123543. 10.3390/cells10123543 PubMed 34944051
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