AAV-EFS-LSL-nlsGFP-shRNA
(Plasmid
#175584)
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Purpose(Empty Backbone) Conditional express shRNA with control of LoxP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175584 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV
- Backbone size (bp) 6148
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Vector typeMammalian Expression, AAV, Cre/Lox
- Promoter EFS
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-EFS-LSL-nlsGFP-shRNA was a gift from Christian Wolfrum (Addgene plasmid # 175584 ; http://n2t.net/addgene:175584 ; RRID:Addgene_175584) -
For your References section:
snRNA-seq reveals a subpopulation of adipocytes that regulates thermogenesis. Sun W, Dong H, Balaz M, Slyper M, Drokhlyansky E, Colleluori G, Giordano A, Kovanicova Z, Stefanicka P, Balazova L, Ding L, Husted AS, Rudofsky G, Ukropec J, Cinti S, Schwartz TW, Regev A, Wolfrum C. Nature. 2020 Nov;587(7832):98-102. doi: 10.1038/s41586-020-2856-x. Epub 2020 Oct 28. 10.1038/s41586-020-2856-x PubMed 33116305