SpRYc
(Plasmid #175575)

• Purpose: Expresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cells
• Depositing Lab: Joseph Jacobson
• Publication: Koseki et al Res Sq. 2023 Mar 7:rs.3.rs-2625838. doi: 10.21203/rs.3.rs-2625838/v1. Preprint.

Backbone

• Vector backbone: Adapted from pCMV-T7-SpRY-P2A-EGFP (Addgene #139989)
• Backbone size w/o insert (bp): 4347
• Total vector size (bp): 8478
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpRYc
• Species: Synthetic
• Insert Size (bp): 4131
• Mutation: PID of SpRY grafted onto the N-terminal domain of Sc++
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • pCMV-T7-SpRYc-P2A-EGFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.researchsquare.com/article/rs-2625838/v1 for preprint.