pX458-Dual-Guide-Donor-Cas9-H2B-mCherry
(Plasmid
#175570)
-
Purpose(Empty Backbone) Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 175570 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepX458
-
Backbone manufacturerFeng Zhang (Addgene plasmid # 48138)
-
Vector typeCRISPR
-
Selectable markersmCherry
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
MutationXbaI site added upstream of U6 promotor. G to C mutation introduced to remove XbaI downstream of U6-Guide casssette.
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pX458-Dual-Guide-Donor-Cas9-H2B-mCherry was a gift from Joseph Brzezinski (Addgene plasmid # 175570 ; http://n2t.net/addgene:175570 ; RRID:Addgene_175570)
