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Addgene

pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt
(Plasmid #175568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 175568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGreenII 0229
  • Vector type
    Plant Expression
  • Selectable markers
    Kanamycin in plants

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GOXL6
  • Species
    A. thaliana (mustard weed)
  • Promoter RUBY
  • Tag / Fusion Protein
    • Citrine (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The plasmid contains a single T nucleotide deletion in the poly T track within GOXL6 compared to the published Col-0 sequence. However, this deletion is in an intron and the resulting fusion with Citrine remains in frame.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt was a gift from George Haughn (Addgene plasmid # 175568 ; http://n2t.net/addgene:175568 ; RRID:Addgene_175568)

  • For your References section:

    Expression Patterns and Functional Characterisation of Arabidopsis GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for Galactose Oxidases in Plants. Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams KL, Haughn GW. Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073. 10.1093/pcp/pcab073 PubMed 34042158
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